Data sheet
| Assay time | 4.5 Hours |
| Specificity | Recombinant and natural ST2/IL-1RL1 |
| Assay Range | 31.2 - 2000 pg/ml |
| Sensitivity | 25 pg/ml |
| Size | 96T |
| Storage | 2 - 8 ℃ |
| Expiration | 6 months |
| Background | Interleukin-1 receptor-like 1 (IL1RL1), also known as protein ST2 (ST2), is a member of the Interleukin-1 receptor family. Human IL1RL1 is synthesized as a 556 amino acid (aa) protein consisting of a 310 (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular Toll/Interleukin-1 Receptor (TIR) domain. Alternative splicing of human IL1RL1 gene generates a soluble isoform without the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig-like domain. The ECD of human IL1RL1 shares 68% and 64% aa sequence identity with mouse and rat counterparts, respectively. IL1RL1 is a receptor for IL-33, a pro-inflammatory IL-1 family cytokine which is constitutively expressed in smooth muscle and airway epithelia. IL-33 is induced by inflammatory stimulation in these cells, keratinocytes, and dermal fibroblasts and by mechanical strain in cardiac fibroblasts. IL-33 binding induces the association of transmembrane IL1RL1 with IL-1 R AcP, a shared subunit which is also used by IL-1 RI and IL-1 Rrp2/IL-1 R6. Soluble IL1RL1 functions as a decoy receptor for IL-33 and blocks transmembrane IL1RL1-mediated IL-33 signaling. In addition to its role in promoting mast cell and Th2 dependent inflammation, transmembrane IL1RL1 activation enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. The elevated levels of soluble IL1RL1 in the serum may be implicated in certain inflammatory conditions, such as allergic asthma, sepsis, trauma, dengue fever, and pulmonary disease. |
| Cross Reactivity | No significant cross reactivity was observed. |
| Assay principle | Sandwich ELISA |
| Dilution Factor | 1 to 10 |
| Sample | 10 µl serum or plasma |
| Specificity | Inter-assay--less than 10.1%; Intra-assay--less than 7.4% |
| Recovery | 88% - 108% |
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