Antibody Services (NB-SC102)

Generation of Stable Cell Lines Expressing Antibodies

Novatein Biosciences' scientists are highly experienced in antibody and cell line development services. We have >95% success rate of stable cell line generation. Stable cell lines (CHO/ HEK293) are generated using our proprietary mammalian expression vector system with multiple selectable markers to simultaneously express two individual genes of interest. After transfection into mammalian cells, the clones are screened for the expression of protein of interest. The standard method for screening clones is ELISA. Additional fee will be charged for Western blot or cell-based assay of your interest. Our fast and predictive screening techniques significantly increase the speed of stable cell line development while maintaining quality and uniformity.

Following will be performed as part of this service:

• Selection of clones
• Detailed characterization of the top clone in a small-scale production system (viability, cell growth, and titer measurements)
• Banking of 10-15 vials of the top clone (upto 10 million cells per vial)

Turnaround: 7-8 weeks.

Antibody Sequencing/ Recombinant Antibody Production

Novatein Biosciences provides comprehensive monoclonal antibody cloning and antibody sequencing service: The antibody (IgG, IgM, IgA, or IgE) variable regions of the heavy chain (VH) and the light chain (VL) from mouse, rat, hamster, rabbit, monkey, or human hybridomas or clonal B cells are PCR amplified and sequenced. To be 100% sure about the results of PCR-based hybridoma antibody cloning and antibody sequencing, antigen-antibody binding is confirmed by transiently expressing recombinant single-chain antibodies (scFv) or whole IgGs in HEK 293 or CHO cells, and testing whether the recombinant scFv or whole IgG retains the antigen binding affinity and specificity.

100000 cells per hybridoma are sufficient for cloning and sequencing of antibody genes.

General procedure:

1) RNA extraction from hybridoma or clonal B cells;

2) cDNA synthesis

3) 5’ RACE amplification using constant regions of V_H and V_L

4) Cloning of PCR fragments

5) Sequencing

7) CDR analysis

Turnaround: 2-3 weeks

Antibody Conjugation Services

Scientists at Novatein Biosciences are experts in antibody conjugation. You supply us the antibody and we conjugate with any molecule you want:

• HRP
• Biotin
• FITC

In addition, we will validate the antibody conjugate using ELISA or western blot.

Turnaround: Less than a week