Data sheet
| Storage | 2 - 8 ℃ |
| Background | Myoglobin is an iron- and oxygen-binding protein found in the muscle tissue of vertebrates in general and in almost all mammals. It is related to hemoglobin, which is the iron- and oxygen-binding protein in blood, specifically in the red blood cells. Myoglobin belongs to the globin superfamily of proteins, and as with other globins, consists of eight alpha helices connected by loops. Human globin contains 154 amino acids. In humans, myoglobin is only found in the bloodstream after muscle injury. It is an abnormal finding, and can be diagnostically relevant when found in blood. Myoglobin is a sensitive marker for muscle injury, making it a potential marker for heart attack in patients with chest pain. |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric |
| Assay Time | ~ 90 min |
| Sample Type | Serum, Plasma, urine |
| Assay Range | 0 - 1000 ng/ml |
| Sensitivity | 5 ng/ml |
| Specificity | 96% |
| Sample Volume | 20 uL |
| Dilution Factors | 1 to 10 |
| Gene ID | 4151 |
| UniProt ID | P02144 |
| Clinical indications | Myoglobin is released from damaged muscle tissue (rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute kidney injury. It is not the myoglobin itself that is toxic (it is a protoxin) but the ferrihemate portion that is dissociated from myoglobin in acidic environments (e.g., acidic urine, lysosomes). Myoglobin is a sensitive marker for muscle injury, making it a potential marker for heart attack in patients with chest pain. However, elevated myoglobin has low specificity for acute myocardial infarction (AMI) and thus CK-MB, cardiac Troponin, ECG, and clinical signs should be taken into account to make the diagnosis. |
More info
Kit Components
1. Standard set: 6 vials
2. One 96-well plate precoated with antibody
3. Assa diluent buffer: 1 vial
4. Detection antibod-HRP conjugate: 1 vial
5. 25 x wash buffer:1bottle
6. TMB substrate: 1 vial
7. TMB stop solution: 12 ml.
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