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Human D-dimer ELISA Kit
BG-HUM10746
Human D.Dimer ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction Competition ELISA kit
Data sheet
| Assay time | 4.5 Hours |
| Validity | 6 months |
| Assay Range | 6.25 - 400 ng /ml |
| Size | 96T |
| Storage | 2 - 8 ℃ |
| Background | D-dimer (or D dimer) is a fibrin degradation product (or FDP), a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis. It is so named because it contains two D fragments of the fibrin protein joined by a cross-link. D-dimers are not normally present in human blood plasma, except when the coagulation system has been activated, for instance because of the presence of thrombosis or disseminated intravascular coagulation. The D-dimer assay depends on the binding of a monoclonal antibody to a particular epitope on the D-dimer fragment. Several detection kits are commercially available; all of them rely on a different monoclonal antibody against D-dimer. For some of these, the area of the D-dimer to which the antibody binds is known. The binding of the antibody is then measured quantitatively by one of various laboratory methods. |
| Assay principle | Sandwich ELISA |
| Standard | Purified from human plasma |
| Applications | Cardiac Biomarker |
More info
D-Dimer indications:
D-dimer testing is of clinical use when there is a suspicion of deep venous thrombosis (DVT), pulmonary embolism (PE) or disseminated intravascular coagulation (DIC). It is under investigation in the diagnosis of aortic dissection.
For DVT and PE, there are possible various scoring systems that are used to determine the a priori clinical probability of these diseases; the best-known is the Wells score.
For a very high score, or pretest probability, a D-dimer will make little difference and anticoagulant therapy will be initiated regardless of test results, and additional testing for DVT or pulmonary embolism may be performed.
For a moderate or low score, or pretest probability:
A negative D-dimer test will virtually rule out thromboembolism: the degree to which the D-dimer reduces the probability of thrombotic disease is dependent on the test properties of the specific test used in the clinical setting: most available D-dimer tests with a negative result will reduce the probability of thromboembolic disease to less than 1% if the pretest probability is less than 15-20%. Chest computed tomography (CT angiography) should not be used to evaluate pulmonary embolism for persons with negative results of a D-dimer assay. A low pretest probability is also valuable in ruling out PE.
If the D-dimer reads high, then further testing (ultrasound of the leg veins or lung scintigraphy or CT scanning) is required to confirm the presence of thrombus. Anticoagulant therapy may be started at this point or withheld until further tests confirm the diagnosis, depending on the clinical situation.aboratory methods.
The following are reference ranges for D-dimer:
|
Units |
Nonpregnant |
First trimester |
Second trimester |
Third trimester |
|
mg/L or µg/mL |
< 0.5 |
0.05 - 0.95 |
0.32 - 1.29 |
0.13 -1.7 |
|
µg/L or ng/mL |
< 500 |
50 - 950 |
320 - 1290 |
130 - 1700 |
|
nmol/L |
< 2.7 |
0.3 - 5.2 |
1.8 - 7.1 |
0.7 - 9.3 |
D-dimer increases with age. It has therefore been suggested to use a cutoff equal to patient’s age in years × 10 µg/L (or x 0.056 nmol/L) for patients aged over 50 years for the suspicion of venous thromboembolism (VTE), as it decreases the false positive rate without substantially increasing the false negative rate.
An alternative measurement of D-dimer is in fibrinogen equivalent units (FEU). The molecular weight of the fibrinogen molecule is about twice the size of the D-dimer molecule, and therefore 1.0 mcg/mL FEU is equivalent to 0.5 mcg/mL of d-dimer.
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