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Human N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA Kit
Human N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA Kit For research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit
Data sheet
| Assay time | 4.5 Hours |
| Specificity | Specific to recombinant and native NT-proBNP |
| Sensitivity | 10.1 pg/ml |
| Size | 96T |
| Storage | 2 - 8 ℃ |
| Expiration | 6 months |
| Application | Research use to measure human NT-proBNP level in plasma and serum samples |
| Background | The N-terminal prohormone of brain natriuretic peptide (NT-proBNP or BNPT) is a prohormone with a 76 amino acid N-terminal inactive protein that is cleaved from the molecule to release brain natriuretic peptide. Both BNP and NT-proBNP levels in the blood are used for screening, diagnosis of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome. The plasma concentrations of both BNP and NT-proBNP are also typically increased in patients with asymptomatic or symptomatic left ventricular dysfunction and is associated with coronary artery disease and myocardial ischemia. |
| Assay Range | 31.2 - 2000 pg/mL |
| Recovery | 94 % - 108% |
| Precision | Inter-assay-- less than 8.3%; Intra-assay-- less than 4.6% |
| Gene ID | 4879 |
| UniProt ID | P16860 |
More info
Human N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA Kit
Reference values
https://www.mayocliniclabs.com/test-catalog/Clinical+and+Interpretive/84291
Under 50 years of age:
N-terminal pro brain natriuretic peptide (NT-proBNP) values below 300 pg/mL have a 99% negative predictive value for excluding acute congestive heart failure (CHF). A cutoff of 1,200 pg/mL for patients with an estimated glomerular filtration rate (eGFR) below 60 yields a diagnostic sensitivity and specificity of 89% and 72% for acute CHF. NT-PROBNP values greater than 450 pg/mL are consistent with CHF in adults under 50 years of age.
50-75 years of age:
NT-proBNP values below 300 pg/mL have a 99% negative predictive value for excluding acute CHF. A cutoff of 1,200 pg/mL, for patients with an eGFR below 60 yields a diagnostic sensitivity and specificity of 89% and 72% for acute CHF. A diagnostic NT-PROBNP cutoff of 900 pg/mL has been suggested in adults 50 to 75 years of age in the absence of renal failure.
Over 75 years of age:
NT-proBNP values below 300 pg/mL have a 99% negative predictive value for excluding acute CHF. A cutoff of 1,200 pg/mL for patients with an eGFR below 60 yields a diagnostic sensitivity and specificity of 89% and 72% for acute CHF. A diagnostic NT-PROBNP cutoff of 1,800 pg/mL has been suggested in adults over 75 years of age in the absence of renal failure.
NT-ProBNP levels are loosely correlated with New York Heart Association (NYHA) functional class (see Table).
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Interpretive Levels for CHF |
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|
Functional Class |
5th to 95th Percentile |
Median |
|
I |
31-1,110 pg/mL |
377 pg/mL |
|
II |
55-4,975 pg/mL |
1,223 pg/mL |
|
III |
77-26,916 pg/mL |
3,130 pg/mL |
|
IV |
* |
* |
|
*In a Mayo Clinic study of 75 patients with CHF, only 4 were characterized as Class IV. Accordingly, range and median are not provided. |
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For protocols and availability questions, please fill out our contact us form and make sure to include your company name, business email, and phone number. We will contact you within 24 hours.
Different lot may vary, please follow the instruction coming with the kit.
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