NLS-dCas9-NLS
Catalog # PR-137213B
$286.00
Recombinant dCas9 (D10A & H840A) protein is suitable for use in imaging of genomic oci in living cells and fixed cells as well as for gene expression regulation.
For molar concentration and other related production information, please refer to:
Nuclease deficient Cas9: Possess no cleavage activity due to D10A and H840A mutations. Has the ability to bind to DNA.
1. Deactivated CRISPR Associated Protein 9 for Minor-Allele Enrichment in Cell-Free DNA Amin Aalipour et al. Clinical Chemistry 2018 Vol. 64, Issue 2 p307-p916
2. Purified Cas9 Fusion Proteins for Advanced Genome Manipulation Jovan Mircetic et al. Small Methods 2017 1, 1600052
3. Disruptive non-disruptive applications of CRISPR/Cas9 Jonathan LSchmid-Burgk Current Opinion in Biotechnology December 2017, Volume 48 Pages 203-209
4. Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins Toshitsugu Fujita et al. Genes to Cells Volume 21, Issue 4 April 2016 Pages 370–377
5. High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding Evan A Boyle et al. PNAS 2017 May, 114 (21) 5461-5466
6.CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells Wulan Deng et al. PNAS 2015 vol. 112 | no. 38 p11870-1187
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Data sheet
- Size
- 50 µg, 313 pmo, with Reaction Buffer
- Storage
- ‐70°C. Avoid freeze thaw cycles.
- Background
- The functions of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and CRISPR-associated (Cas) genes are essential in adaptive immunity in select bacteria and archaea. CRISPR uses a Cas9 protein to recognize DNA sequences, with target spe
- Purity
- Greater than 90% as determined by SDS-PAGE.
- Host/Source
- E.coli
- Applications
- Recombinant dCas9 (D10A & H840A) protein is suitable for use in imaging of genomic oci in living cells and fixed cells as well as for gene expression regulation.
- Sequence
- Mutated CRISPR-associated endonuclease Cas9 (amino acids 1 to 1368) with D10A & H840A (Uniprot: Q99ZW2 for Cas9). To facilitate nuclear entry, two nuclear localization signal sequence (NLS) are fused to both N- and C-terminal of dCas9 protein.
- Concentration
- 1 µg/ µL, 6.26 µM
- Storage buffer
- 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and
50% (v/v) Glycerol.
- Pysical Appearance
- Sterile filtered colorless solution.
- Storage and Stability
- Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C
for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or
BSA).
Avoid multiple freeze-thaw cycles.
- Formulatin
- Recombinant dCas9 (D10A & H840A) protein expressed in E. coli supplied in a buffer of 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
New
Recombinant dCas9 (D10A & H840A) protein is suitable for use in imaging of genomic oci in living cells and fixed cells as well as for gene expression regulation.