Cas9 monoclonal antibody, biotinylated

Data sheet

Size

25 µg

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Biotinylated antibodies are designed to be detected using streptavidin or anti-biotin antibody conjug

Host/Source

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to Cas9 from Streptococcus pyogene.

Description

This Cas9 antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cas9 Mouse mAb.

Dilution & Recommended Usage

Western blotting 1:1000, ELISA: 1:2000 to 1:8000

Introdcution

The CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the Streptococcus pyogenes CRISPR antiviral immunity system that provides adaptive immunity against extra chromosomal genetic material. The CRISPR antiviral mechanism of acti

Specificty

Cas9 (7A9-3A3) Mouse mAb (Biotinylated) recognizes transfected levels of total Cas9 protein.