Biotinylated NLS-dCas9-NLS

Catalog # PR-137213-bio

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$286.00
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For molar concentration and other related product information, please click here:

CRISPR/Cas9 related enzyme proteins, antibodies, and other reagents

 

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Data sheet

Size
50 µg, 313 pmol
Storage
‐70°C. Avoid freeze thaw cycles.
Application
Recombinant dCas9 (D10A & H840A) protein is suitable for use in imaging of genomic loci in living cells and fixed cells as well as for gene expression regulation.
Background
The functions of CRISPR and CRISPR-associated (Cas) genes are essential in adaptive immunity in some bacteria and archaea. CRISPR uses a Cas9 protein to recognize DNA sequences, with target specificity solely determined by a small guide (sg) R
Formulation
Recombinant dCas9 (D10A & H840A) protein expressed in E. coli supplied in a buffer of 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Host/Source
E. coli
Sequence
Mutated CRISPR-associated endonuclease Cas9 (amino acids 1 to 1368) with D10A
& H840A (Uniprot: Q99ZW2 for Cas9)
Amino Acids
D10A/H840A
Concentration
1 µg/ µL, 6.26 µM
Physical appearance
Sterile filtered colorless solution.
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