NLS-Cas9-NLS
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  • NLS-Cas9-NLS

NLS-Cas9-NLS

Catalog # PR-137211

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$480.00

Cas9 NLS, S. pyogenes contains Simian virus 40(SV40) T agtigen nuclear logcalization sequence( NLS) on the N- and C- termini of the protein.

Quantity

For molar concentration and related product information, please refer to: CRISPR/Cas9 related enzyme proteins, antibodies, and other reagents



In vitro Cas9 mediated digestion of DNA

1. Add the following components to a sterile, nuclease-free tube on ice:

Components

Volume

Final Concentration

  

sgRNA (300 nM)

2 µl

~30 nM

  

Cas9 Nuclease Protein

(1000 nM)

0.60 µl

~30 nM

  

10X Cas9 Nuclease Reaction

Buffer

2 μl

1X

  

Nuclease-free H2O

12.4 μl

-

  

Pre-Incubate for 15 minutes at room temperature

  

Substrate DNA (30 nM)

3 μl

3 nM

  

Total Volume

20 μl

-

  

 

2. Collect all components by a brief centrifugation. Incubate the reaction at 37 °C for 1 hour.

3. Analyze fragments by running an agarose gel electrophoresis.

Related Products

NLS-Cas9-NLS     NLS-Cas9-EGFP                 NLS-Cas9(D10A)-2NLS nickase 

dCas9 (D10A & H840A)       NLS-dCas9-NLS        Biotinylated NLS-dCas9-NLS

dCas9-SBP       NLS-dCas9 -EGFP-NLS               LbCas12a (Cpf1) Nuclease

eSpCas9(1.1)-3NLS              SpCas9-HF1-3NLS         Cas9 ELISA kit

Cas9 Monoclonal Antibody    Cas9 monoclonal antibody, biotinylated

Data sheet

Size
50 µg, 313 pmol, with Reaction Buffer
Formulation
Recombinant Cas9 wild type protein expressed in E. coli supplied in a buffer of 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Host/Source
E.coli
Applications
Create double blunt end for recombination
Storage Conditions
-70°C. Avoid freeze thaw cycles.
Concentration
1 µg/ µL, 6.26 µM
Storage buffer
10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Reaction Conditions
Use 1X Cas9 Reaction Buffer and incubate at 37 °C.
Physical appearance
Sterile filtered colorless solution.
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