Biotinylated NLS-dCas9-NLS
Catalog # PR-137213-bio
$286.00
For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
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Data sheet
- Size
- 50 µg, 313 pmol
- Storage
- ‐70°C. Avoid freeze thaw cycles.
- Application
- Recombinant dCas9 (D10A & H840A) protein is suitable for use in imaging of genomic loci in living cells and fixed cells as well as for gene expression regulation.
- Background
- The functions of CRISPR and CRISPR-associated (Cas) genes are essential in adaptive immunity in some bacteria and archaea. CRISPR uses a Cas9 protein to recognize DNA sequences, with target specificity solely determined by a small guide (sg) R
- Formulation
- Recombinant dCas9 (D10A & H840A) protein expressed in E. coli supplied in a buffer of 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
- Host/Source
- E. coli
- Sequence
- Mutated CRISPR-associated endonuclease Cas9 (amino acids 1 to 1368) with D10A
& H840A (Uniprot: Q99ZW2 for Cas9)
- Amino Acids
- D10A/H840A
- Concentration
- 1 µg/ µL, 6.26 µM
- Physical appearance
- Sterile filtered colorless solution.
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