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SMAD 1/2/3/5 Antibody View larger

SMAD 1/2/3/5 Antibody

ASA-B1723

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
SMAD family member 1 Polyclonal IgG Rabbit Human, Mouse, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SMAD 1/2/3/5(442-456aa GPLQWLDKVLTQMGS), identical to the related mouse and rat sequences.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 SMAD1

Protein

 Mothers against decapentaplegic homolog 1(MAD homolog 1/Mothers against DPP homolog 1)

Uniprot ID

 Q15797

Function

 Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

Tissue Specificity

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Sub-cellular localization

 Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.

Sequence Similarities

 Belongs to the dwarfin/SMAD family.

Aliases

 BSP 1 antibody|BSP1 antibody|DKFZP586N0721 antibody|DKFZp686J10186 antibody|Dwarfin A antibody|DwfA antibody|hMAD 2 antibody|hMAD 3 antibody|hSMAD2 antibody|hSMAD3 antibody|hSmad5 antibody|HSPC193 antibody|HST17436 antibody|JV15 2 antibody|JV152 antibody|JV18 1 antibody|JV18 antibody|JV181 antibody|JV4 1 antibody|MAD(mothers against decapentaplegic Drosophila) homolog 3 antibody|MAD antibody|MAD homolg 5 antibody|MAD mothers against decapentaplegic homolog 1 antibody|Mad related protein 1 antibody|MAD Related Protein 2 antibody|MAD3 antibody|MADH 1 antibody|MADH 3 antibody|MADH1 antibody|MADH2 antibody|MADH3 antibody|MADR1 antibody|MADR2 antibody|MGC22139 antibody|MGC34440 antibody|MGC60396 antibody|Mothers against decapentaplegic homolog 1 antibody|Mothers Against Decapentaplegic Homolog 2 antibody|Mothers against decapentaplegic homolog 3 antibody|Mothers against decapentaplegic homolog 5 antibody|Mothers against DPP homolog 1 antibody|Mothers against DPP homolog 2 antibody|Mothers against DPP homolog 3 antibody|Mothers against DPP homolog 5 antibody|Sma and Mad related protein 1 antibody|SMA and MAD related protein 3 antibody|SMAD antibody|SMAD 1 antibody|SMAD 2 antibody|SMAD 3 antibody|SMAD 5 antibody|SMAD family member 1 antibody|SMAD mothers against DPP homolog 1 Drosophila antibody|Spinal muscular atrophy distal with upper limb predominance antibody|TGF beta signaling protein 1 antibody|Transforming growth factor beta signaling protein 1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SMAD 1/2/3/5 antibody, ASA-B1723, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: MM453 Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: SMMC Cell Lysate
Lane 9: SW620 Cell Lysate

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