More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prominin 1 | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse PROM1(850-864aa KDHLYGVHNPVMTSP), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PROM1 |
Protein | Prominin-1 |
Uniprot ID | O54990 |
Function | May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
Tissue Specificity | In the submandibular gland, expressed on the apical side of epithelial cells. In the parotid gland, expressed in the intercalated ducts. In the sublingual gland, expressed in intercalated ducts. In the extraorbital lacrimal gland, expressed in the intercalated tubules and larger intralobular ducts. Expressed in the retina. Present in urine within small membrane particles (at protein level). In the embryo, expressed on the apical side of neuroepithelial cells and of other epithelia such as lung buds, gut and ureter buds. In the adult, expressed at the apical side of the kidney tubules and of the ependymal layer of the brain. Not expressed in gut, liver, lung, pituitary, adrenal, heart or spleen. Localized to the nascent disk membranes at the base of the rod outer segment in the retina (at protein level). |
Sub-cellular localization | Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum . Endoplasmic reticulum-Golgi intermediate compartment . Note: Found in extracellular membrane particles in various body fluids such as ventricular fluid of the developing brain and urine. |
Sequence Similarities | |
Aliases | AC133 antibody|Antigen AC133 antibody|CD133 antibody|CORD12 antibody|hematopoietic stem cell antigen antibody|hProminin antibody|MCDR2 antibody|MSTP061 antibody|PROM1 antibody|PROM 1 antibody|PROM-1 antibody|Prominin-1 antibody|Prominin 1 antibody|Prominin1 antibody|Prominin-like protein 1 antibody|PROML1 antibody|RP41 antibody|STGD4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PROM1 antibody, ASA-B1578, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 4: Mouse Kidney Tissue Lysate
Lane 5: Mouse Testis Tissue Lysate
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 4: Mouse Kidney Tissue Lysate
Lane 5: Mouse Testis Tissue Lysate
