Protein Kinase Akt1/PKB alpha, Inactive enzyme Human Recombinant ( PKAkt1/PKBa Human )

Catalog # PT_75012

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$590.00
DescriptionPKAkt1 is a glycosilated polypeptide having a molecular mass of 59.1 kDa, fused with a polyhistidine tag at N-terminus (to facilitate removal of Akt1 kinase from the reaction mixture).Inactive enzyme, suitable for negative control
Product Size: 5ug
Quantity

Data sheet

Formulation
PKAkt1 1.9mg/ml, in 50mM NaCl, 1mM DTT, 25mM beta glycerophosphate, 50% glycerol, pH 8.5.
Purity
Greater than 90% as determined by SDS-PAGE.
Description
Recombinant Human Protein Kinase B is a glycosylated polypeptide having a molecular mass of 59.1 kDa. Recombinant Protein Kinase B is purified by proprietary chromatographic techniques.
Protein Background
Akt1, also known as "Akt" or protein kinaseB (PKB) is an important molecule in mammaliancellular signaling.In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. These enzymesare members of the serine/threonine-specific protein kinase
Expression host
Sf9 insect cells.
Synonyms
RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, AKT1, AKT, RAC, PRKBA, MGC99656, RAC-ALPHA.
Reagent Appearance
Sterile Filtered clear solution.
Unit Definition
20.000 Units/mg (1 Unit = 1 pmol/min transferred to synthetic peptide RPRAATF at 30 degree Celsius). No protease activity detectable.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Activity
No protease activity (Twinning test). The Specific activity is 235 U/mg.
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