Mitogen-Activated Protein Kinase 3 Human Recombinant, His-Tag ( MAPK3 Human, His )

Data sheet

Formulation

The MAPK3 0.5mg/ml protein solution contains 20mM Tris-HCl buffer pH-8, 1mM DTT, 0.1M NaCl and 20% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

MAPK3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 399 amino acids (1-379 a.a.) and having a molecular mass of 45.2 kDa. The MAPK3 is fused to a 20 amino acid His-Tag at N-terminus and purified by propri

Protein Background

MAPK3 is a part of the MAP kinase family which is recognized as extracellular signal-regulated kinases (ERKs), that function in a signaling cascade that controls various cellular procedures such as proliferation, differentiation, and cell cycle progressio

Expression host

Escherichia Coli.

Synonyms

ERK1, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAP kinase3.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF LTEYVATRWY RA

Physical Appearence

Sterile Filtered colorless solution.