Protein Kinase C alpha Human Recombinant ( PKC-a Human )

Data sheet

Purity

Greater than 95% as determined by SDS-PAGE.

Description

Protein Kinase C alpha Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-672 and having a molecular mass of 76.7 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag.PKC-

Protein Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved

Expression host

Sf9 insect cells.

Synonyms

Protein kinase C alpha type, EC 2.7.11.13, PKC-alpha, PKC-A, PRKCA, AAG6, PKCA, PRKACA, MGC129900, MGC129901.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Amino acid sequence

MADVYPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LK

Unit Definition

7200- 9000 units/mg. One unit of PKC-a activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC-a also phosphorylates full length myelin basic protein, histone, MARKS,

Specific Activity

1570 nmole of phosphate transferred to Histone H1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 2ug/ml.

Caution

The Sale of recombinant Human PTEN by ProSpec is prohibited in the following countries: United States, Japan, Australia, Canada, Austria, Belgium, Denmark, Finland, France, Germany, Ireland, Italy, Liechtenstein, Luxembourg, Netherlands, Portugal, Spain,