Catalog # NP-HS10041
|
Assay Range |
0.78--50 pg/mL |
|
Sensitivity |
0.25 pg/mL |
|
Specificity |
No cross-reaction with other related substances detected |
|
Size |
96T |
|
Storage |
Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples |
|
Sample Type |
serum, plasma or cell culture supernatant |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Mouse IL-17 standard: 50 pg x 2 vials
2. One 96-well plate coated with Mouse IL-17 Ab
3. Sample diluent buffer: 12 mL - 2
4. Detection antibody: 60 µL, 1:180
5. Streptavidin-HRP: 300 µL, 1:40
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 6 ml TMB x1, 6 ml H2O2 x1.
9. Stop solution: 6 mL x1
10. Washing solution (20x): 25 mL x1
Data sheet
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