Mouse IL-4 ELISA Kit-High Sensitivity
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  • Mouse IL-4 ELISA Kit-High Sensitivity

Mouse IL-4 ELISA Kit-High Sensitivity

Catalog # NR-HS10594

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$625.00
Quantity

 

Size
96T
 
Range
0.78 pg/mL-50 pg/mL
 
Sensitivity
0.24 pg/mL
 
Specificity
No detectable cross-reactivity with any other cytokine.
 
Storage
Store at 4 °C for frequent use, at -20 °C for infrequent use.
 
 
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 
Expiration
6 months at 4 °C and 12 months at -20 °C.
 
Application
serum, body fluids, tissue lysate, cell culture supernates.
 
 
 
 
Kit Components
 
 
 
1. Lyophilized recombinant mouse IL-4 standard: 50 pg/tube-2.
 
 
2. One 96-well plate precoated with anti- mouse IL-4 antibody.
 
 
3. Sample diluent buffer: 12 ml x 2
 
 
4. Biotinylated anti- mouse IL-4 antibody: 60 µL, dilution 1:180.
 
 
5. Antibody diluent buffer: 12ml.
 
 
6. Strepavidin-HRP: 300µL, dilution 1:40.
 
 
7. Strepavidin-HRPdiluent buffer: 12ml.
 
 
8. Chromogen-A, 6 ml; Chromogen-B, 6 ml.
 
 
9. Stop Solution: 6 ml.

Data sheet

Assay Range
0.78 – 50 pg/mL
Sensitivity
0.25 pg/mL
Background
The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produce
Sample
5-10 µL mouse serum/plasma
Alternate Names
BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
Gene ID
16189
UniProt ID
P07750
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