Small Nuclear Ribonucleoprotein Polypeptide D3 Human Recombinant, Sf9 ( SNRPD3 Human, Sf9 )

Data sheet

Formulation

SNRPD3 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Purity

Greater than 80% as determined by SDS-PAGE.

Applications

Western blot with anti-Sm positive patient sera.

Description

SNRPD3 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 14,739 Dalton. SNRPD3 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Protein Background

SNRPD3 is a small nuclear ribonucleoprotein (snRNPs) that contains the spliceosome in eukaryotes. SNRPD3 is essential for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing happens in this locus and two transcript varia

Expression host

Sf9 insect cells.

Synonyms

Small nuclear ribonucleoprotein D3 polypeptide 18kDa, Sm-D3, snRNP core protein D3.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Coating concentration

0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.