Ras Homolog Gene Family Member B Human Recombinant ( RHOB Human )

Data sheet

Formulation

RHOB protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 20% glycerol and 0.2mM NaCl.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

RHOB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-193 a.a.) and having a molecular mass of 23.9kDa (Molecular weight on SDS-PAGE will appear higher).RHOB is fused to a 20 amino acid His-tag at N-terminus

Protein Background

Rho-related GTP-binding protein RhoB (RhoB) belongs to the Rho GTP-binding protein family. RhoB facilitates apoptosis in neoplastically transformed cells after DNA damage. RhoB has a negative role in tumorigenesis since deletion causes tumor formation.

Expression host

Escherichia Coli.

Synonyms

Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6, RHOB, ARH6, ARHB, RHOH6, MST081, MSTP081.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC.