Nucleopurin 62kDa Human Recombinant ( NUP62 Human )

Data sheet

Formulation

NUP62 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Purity

Greater than 80% as determined by SDS-PAGE.

Applications

Western blot with:a) Polyclonal anti-Nup62 antibodies.b) Monoclonal anti-hexa-His-tag antibody.

Description

NUP62 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 54,626 Dalton. NUP62 is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.

Protein Background

Nucleopurin 62kDa (NUP62) belongs to the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. Nucleoporins are the principal components of the nuclear pore complex in eukaryotic cells. This complex is a colossal structure w

Expression host

Sf9 insect cells.

Synonyms

Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62, p62, IBSN, SNDI.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Coating concentration

0.5-1.0 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.