Cytochrome B5 Reductase 3 Human Recombinant ( CYB5R3 Human )

Data sheet

Formulation

The CYB5R3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

CYB5R3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 300 amino acids (27-301) and having a molecular mass of 34.0kDa.CYB5R3 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chrom

Protein Background

CYB5R3 is a cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplas

Expression host

E.coli.

Synonyms

Cytochrome b5 reductase 3, DIA1, B5R, Diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, Diaphorase-1, EC 1.6.2.2.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMFQRST PAITLESPDI KYPLRLIDRE IISHDTRRFR FALPSPQHIL GLPVGQHIYL SARIDGNLVV RPYTPISSDD DKGFVDLVIK VYFKDTHPKF PAGGKMSQYL ESMQIGDTIE FRGPSGLLVY QGKGKFAIRP DKKSNPIIRT VKSVGMIAGG TGITPMLQVI RAIMKDPDDH TVCHLLFANQ TEKDILLRPE LEELRNKHSA RF