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Data sheet
| Formulation | AP1S2 protein solution (0.5mg/1ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.1M NaCl and 2mM DTT. |
| Purity | Greater than 85.0% as determined by SDS-PAGE. |
| Description | AP1S2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 177 amino acids (1-157a.a) and having a molecular mass of 20.7kDa.AP1S2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | AP1S2 belongs to the adaptin protein family and functions as the small subunit of AP-1 complex 1 which is found in the cytoplasmic face of coated vesicles placed at the Golgi complex. AP-1 complex 1 facilitates both the necessity of clathrin to the membrane and the identification of organizing signals within the cytosolic tails of transmembrane receptors. |
| Expression host | Escherichia Coli. |
| Synonyms | Adaptor-related protein complex 1 sigma 2 subunit, SIGMA1B, Adaptor protein complex AP-1 sigma-1B subunit, Clathrin assembly protein complex 1 sigma-1B small chain, Golgi adaptor HA1/AP1 adaptin sigma-1B subunit, Sigma 1B subunit of AP-1 clathrin, MRX59, mental retardation X-linked 59, MGC:1902, AP-1 complex subunit sigma-2, golgi adaptor HA1/AP1 adaptin sigma 1B subunit. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Stability | AP1S2 although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MQFMLLFSRQ GKLRLQKWYV PLSDKEKKKI TRELVQTVLA RKPKMCSFLE WRDLKIVYKR YASLYFCCAI EDQDNELITL EIIHRYVELL DKYFGSVCEL DIIFNFEKAY FILDEFLLGG EVQETSKKNV LKAIEQADLL QEEAETPRSV LEEIGLT |
More info
Adaptor protein complex AP-1 sigma-1B subunit, AP-1 complex subunit sigma-2, Clathrin assembly protein complex 1 sigma-1B small chain, golgi adaptor HA1/AP1 adaptin sigma 1B subunit, Golgi adaptor HA1/AP1 adaptin sigma-1B subunit, mental retardation X-linked 59, MGC:1902, MRX59, Sigma 1B subunit of AP-1 clathrin, SIGMA1B, Adaptor-related protein complex 1 sigma 2 subunit