Angiostatin Kringles 1-3 Human Recombinant ( Ang K1-3 Human )

Data sheet

Formulation

Lyophilized from a 0.2m filtered concentrated (1.0mg/ml) solution in 20mM NaAc, pH5.5, 4% mannitol.

Solubility

We recommend to briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into

Purity

Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Angiostatin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 259 amino acids and having a molecular mass of approximately 30.0 kDa. The Ang K1-3 is purified by proprietary chromatographic techniques.

Protein Background

Ang K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4. Ang K1-3 reduces endothelial cell proliferation and ac

Expression host

Escherichia coli.

Synonyms

Angiostatin, Angiostatin Kringles 1-3, Ang K1-3.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

The lyophilized Angiostatin K1-3 is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion t

Amino acid sequence

VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLKGTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGK

Biological Activity

The activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 55,000 Units/mg.

References

Title: Regenerative protein thymosin b
-4 is a novel regulator of purinergic signaling. Publication: Published online before printNovember 24, 2010, doi:10.1096/fj.10-169417 March 2011The FASEB Journalvol. 25no. 3907-915.Link: http://www.fasebj.org/conten

Human Virus Test

The Iron content was estimated by ICP and was found to be 2.0 ppm.

Advantages

Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG and Syphilis.