> >>Copper Chaperone for Superoxide Dismutase Human Recombinant ( CCS Human )
View larger

Copper Chaperone for Superoxide Dismutase Human Recombinant ( CCS Human )

DescriptionCCS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 294amino acids (1-274a.a.) and having a molecular wieght of 31.2kDa. The CCS is fused to 20a.a. His-Tag at N-terminus and purified by

$193.00

Data sheet

Reconstitution 1mg/ml in PBS (after reconstitution).
Formulation The CCS protein solution (1mg/1ml) contains 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT 0.2M NaCl and 10% glycerol.
Purity Greater than 90.0% as determined by SDS-PAGE.
Description CCS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 294amino acids (1-274a.a.) and having a molecular wieght of 31.2kDa. The CCS is fused to 20a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Protein Background CCS is vital for the integration of copper into SOD-1, and as a result is needed for its enzymatic activity. CCS inhibits copper ions from binding to intracellular copper scavengers and provides the SOD-1 enzyme with the required copper cofactor. CCS escorts copper just to SOD-1 and is unable to deliver copper to proteins in the mitochondria, nucleus or secretory pathway. Although many tissues express CCS, the chaperone is most abundant in the kidney, liver and Purkinje cells in the neuropil of the central nervous system.
Expression host Escherichia Coli.
Reagent Appearance Sterile filtered colorless solution.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MASDSGNQGT LCTLEFAVQM TCQSCVDAVR KSLQGVAGVQ DVEVHLEDQM VLVHTTLPSQ EVQALLEGTG RQAVLKGMGS GQLQNLGAAV AILGGPGTVQ GVVRFLQLTP ERCLIEGTID GLEPGLHGLH VHQYGDLTNN CNSCGNHFNP DGASHGGPQD SDRHRGDLGN VRADADGRAI FRMEDEQLKV WDVIGRSLII DEGEDDLGRG GHPLSKITGN SGERLACGII ARSAGLFQNP KQICSCDGLT IWEERGRPIA GKGRKESAQP PAHL
Antibody Properties Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Available Conjugates Staining antibody. For staining, use 5-10ul/1,000,000 cells.
Immunological function This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10ul/106 cells.

© 2024 Novateinbio.com