Centromere Protein B Human Recombinant ( CENPB Human )

Data sheet

Formulation

CENPB is supplied at a concentration of in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Description

CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kDa. CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Protein Background

Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere pr

Expression host

Sf9 insect cells.

Synonyms

Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.

Reagent Appearance

Sterile Filtered liquid formulation.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Coating concentration

0.15-0.375 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.