Cysteine-Rich Secretory Protein 1 Human Recombinant ( CRISP1 Human )

Data sheet

Formulation

The protein was lyophilized from a 0.5mg/ml solution containing 20mM TRIS, 50mM NaCl, pH 7.5.

Solubility

It is recommended to reconstitute the lyophilized CRISP1 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 85% as determined by SDS-PAGE.

Applications

Western blotting, ELISA.

Description

The CRISP1 Protein Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (Lys 22 - Lys 249) having a molecular mass of 27kDa.CRISP1 is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary

Protein Background

Insemination involves of a series of specific cell-cell interactions concluding in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion take place through the interface of complementary molecules which are localized to specif

Expression host

Escherichia Coli.

Synonyms

Cysteine-rich secretory protein 1, ARP, CRISP-1, AEGL1, Acidic epididymal glycoprotein-like 1, HSCRISP1D, HSCRISP1G, HUMARP, AEG-like protein, cysteine-rich secretory protein-1 delta.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized CRISP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CRISP1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Amino acid sequence

MKHHHHHHAS KKKSARDQFN KLVTDLPNVQ EEIVNIHNAL RRRVVPPASN MLKMSWSEEA AQNARIFSKY CDMTESNPLE RRLPNTFCGE NMHMTSYPVS WSSVIGVWYS ESTSFKHGEW TTTDDDITTD HYTQIVWATS YLIGCAIASC RQQGSPRYLY VCHYCHEGND PETKNEPYKT GVPCEACPSN CEDKLCTNPC IYYDEYFDCD IQVHYLGCNH STTILFCKAT CL