Cullin-1 Human Recombinant ( CUL1 Human )

Data sheet

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

Formulation

The CUL1 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 10% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

CUL1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 430 amino acids (1- 410a.a.) and having a molecular mass of 49.4kDa.CUL1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniq

Protein Background

Cullin1, is an essential component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, that facilitate the ubiquitination of proteins which take part in cell cycle progression, signal transduction and transcr

Expression host

Escherichia Coli.

Synonyms

Cullin 1, CUL-1, Cullin-1

Reagent Appearance

Sterile Filtered clear solution.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MSSTRSQNPH GLKQIGLDQI WDDLRAGIQQ VYTRQSMAKS RYMELYTHVY NYCTSVHQSN QARGAGVPPS KSKKGQTPGG AQFVGLELYK RLKEFLKNYL TNLLKDGEDL MDESVLKFYT QQWEDYRFSS KVLNGICAYL NRHWVRRECD EGRKGIYEIY SLALVTWRDC LFRPLNKQVT NAVLKLIEKE RNGETINTRL ISGVVQSYVE LG

link

Purified by proprietary chromatographic technique.