Cytochrome B5 Type A Human Recombinant ( CYB5A Human )

Data sheet

Formulation

CYB5A protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

CYB5A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a) and having a molecular mass of 14.9kDa.CYB5A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chro

Protein Background

Cytochrome b5 isoform 1 (CYB5A) is a member of the cytochrome b5 family. CYB5A is a membrane bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases. Defects in the CYB5A gene are the cause of methemoglobinemia CYB5A

Expression host

Escherichia Coli.

Synonyms

Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAEQSD EAVKYYTLEE IQKHNHSKST WLILHHKVYD LTKFLEEHPG GEEVLREQAG GDATENFEDV GHSTDAREMS KTFIIGELHP DDRPKLNKPP ETLITTIDSS SS.

Protein concentration

Lateral flow immunoassay.

link

Purified monoclonal IgG by protein A chromatography.

Rapid Test Assay

1. Fresh gold particle prepared within 1 to 2 days with maximal homogeneity in particle size, ? 530 to 534, OD 1.2- 1.4.2. Conjugation pH 8.5, conjugation time should be 6-8 hours.3. Blocking condition: 0.4-0.45% casein.