DnaK ( HSP70 ) Mycobacterium Tuberculosis Recombinant ( DnaK Mycobacterium Tuberculosis )

Data sheet

Formulation

The DnaK protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Na-phosphate buffer pH 7.4, 130mM NaCl and 2.5mM KCl.

Solubility

It is recommended to reconstitute the lyophilized DnaK in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Inactivation

EGF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of p

Description

Recombinant Mycobacterium Tuberculosis Dnak produced in E.Coli is a single, non-glycosylated polypeptide chain containing 625 amino acids and having a molecular mass of 66.7 kDa.

Protein Background

DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-de

Expression host

Escherichia Coli.

Synonyms

HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, 70 kDa antigen, ML2496.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized DnaK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DnaK should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ca