Thiol Disulfide Interchange Protein E.Coli Recombinant ( DsbE E.Coli )

Data sheet

Formulation

The DsbE protein solution contains 20mM Tris-HCl, pH-7.5, 2mM EDTA and 10% Glycerol.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Recombinant DsbE produced in E.Coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.1 kDa. DsbE is purified by conventional chromatography techniques.

Protein Background

DsbE is a reducing Dsb protein involved in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. DsbE functions is to decrease disulphide bonds formed a

Expression host

Escherichia Coli.

Synonyms

Thiol:disulfide interchange protein dsbE, Cytochrome c biogenesis protein ccmG, dsbE, ccmG, yejQ, b2195, JW2183.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q.