Eukaryotic Translation Initiation Factor 5A Human Recombinant ( EIF5A Human )

Data sheet

Formulation

The EIF5A protein solution (1mg/ml) contains 50mM Tris-HCl, pH-7.5 and 10% Glycerol.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

EIF5A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 154 amino acids and having a molecular mass of 16.8 kDa.EIF5A is purified by proprietary chromatographic techniques.

Protein Background

EIF5A is the single protein identified to contain remarkable amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. EIF5A takes part in the first step of peptide bond formation in transla

Expression host

Escherichia Coli.

Synonyms

EIF-5A, EIF5A1, eIF5AI, MGC99547, MGC104255, EIF5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-4D, Rev-binding factor.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Amino acid sequence

MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK.

References

Title: Inhibition of Deoxyhypusine Synthase Enhances Islet b Cell Function and Survival in the Setting of Endoplasmic Reticulum Stress and Type 2 DiabetesPublication: Journal of Biological Chemistry 285.51 (2010): 39943-39952.Link: http://www.jbc.org/cont