Coagulation Factor-VIII Human Recombinant ( F8 Human )

Data sheet

Formulation

Each 250IU of lyophilized protein contain 6mg/vial L-histidine, 72mg/vial NaCl, 1mg/vial Calcium Chloride dihydrate, 0.4mg/vial Tween 80, 12mg/vial Sucrose, pH 7.0 (After reconstitution with 4ml of 0.9% Saline).

Solubility

It is recommended to reconstitute 250IU lyophilized Factor-VIII in 4ml of 0.9% Saline, After reconstitution, Factor-VIII concentration will be 62.5IU/ml and should be used within 3 hours.

Purity

Greater than 97.0% as determined by SDS-PAGE.

Description

Antihemophilic Facor Human Recombinant produced in CHO is a glycosylated polypeptide chain having a total amino acids of 1438 (170kd) and consisting of two dimer chains 80 kD and 90 kD.The Factor-VIII lacks central region of B-domain region and is purifie

Protein Background

Coagulation factor VIII participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively

Expression host

CHO cells (Chinese Hamster Ovarian Cells).

Synonyms

Coagulation factor VIII, Procoagulant component, Antihemophilic factor, AHF, F8, F8C, F8B, HEMA, FVIII, DXS1253E, F8 protein.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Factor-VIII although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Factor-VIII should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Biological Activity

The specific activity was found to be 10,169 IU/mg.