GABA ( A ) Receptor-Associated Protein Like 1 Human Recombinant ( GABARAPL1 Human )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The GABARAPL1 solution (1 mg/ml) contains 20mM Tris buffer (pH 8.0), 10% glycerol, 1mM DTT and 0.1M NaCl.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

GABARAPL1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 137 amino acids (1-117 a.a.) and having a molecular mass of 16.2kDa (Molecular size on SDS-PAGE wil

Protein Background

GABARAPL1 [Gamma-aminobutyric acid (GABA) receptor-associated protein-like 1] is a member of the MAP1 LC3 family. GABARAPL1 increases cell surface expression of kappa type opioid receptor through facilitating anterograde intracellular trafficking of the r

Expression host

Escherichia Coli.

Synonyms

Gamma-aminobutyric acid receptor-associated protein-like 1, Early estrogen-regulated protein, GABA(A) receptor-associated protein-like 1, Glandular epithelial cell protein 1, GEC-1, GABARAPL1, GEC1, ATG8, APG8L, ATG8B, ATG8L, APG8-LIKE.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

GABARAPL1 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MKFQYKEDHP FEYRKKEGEK IRKKYPDRVP VIVEKAPKAR VPDLDKRKYL VPSDLTVGQF YFLIRKRIHL RPEDALFFFV NNTIPPTSAT MGQLYEDNHE EDYFLYVAYS DESVYGK.

Antibody Properties

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA ,1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories.