High-Mobility Group Box 1 Human Recombinant, Hi-5 ( HMGB1 Human, Hi-5 )

Data sheet

Formulation

The HMG1 solution (1mg/ml) contains 20mM Tris-HCl pH-8, 1mM EDTA, 0.5mM DTT and 10% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

HMG1 Human Recombinant fused to an 8 aa His-Tag at C-terminus produced in High Five insect cells is a single, glycosylated, polypeptide chain (amino acids 1-215) containing 223 amino acids and having a molecular mass of 25 kDa.The HMGB1 is purified by pro

Protein Background

HMGB1 is an abundant chromatin-binding protein found in eukaryotic cell nucleus and acts in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and is involved in transcriptional regulation. Outside the cell, HMGB1 acts as a cytok

Expression host

Hi-5 Insect Cells.

Synonyms

HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH.