Heat-Responsive Protein 12 Human Recombinant ( HRSP12 Human )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The HRSP12 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

HRSP12 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids (1-137 a.a.) and having a molecular mass of 16.6kDa. The HRSP12 is purified by proprie

Protein Background

HRSP12 (UK114) is an endoribonuclease found mainly in the human adult kidney and liver, and which is responsible for inhibiting translation by cleaving mRNA. HRSP12 cleaves phosphodiester bonds only in single-stranded RNA. HRSP12 may be an important bioma

Expression host

Escherichia Coli.

Synonyms

Ribonuclease UK114, 14.5 kDa translational inhibitor protein, p14.5, Heat-responsive protein 12, UK114 antigen homolog, HRSP12, PSP.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

HRSP12 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL.

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Introductionn

1:1,000 dilution will block T cell proliferation induced by specific Ag or concanavaline A. 10ul will stain 106 IL-R positive cells for FACS analysis.