Heat Shock Protein 104 Saccharomyces cerevisiae Recombinant ( HSP104 Saccharomyces )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The HSP-104 protein solution contains 20mM Tris-HCl, pH 7.4,100mM NaCl, 2mM EDTA and 5% Glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

Recombinant HSP104 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 908 amino acids and having a molecular mass of 102 kDa.

Protein Background

HSP104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation stud

Expression host

Saccharomyces cerevisiae.

Synonyms

Heat shock protein 104, Protein aggregation-remodeling factor HSP104, HSP104, YLL026W, L0948.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:5,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).