Heat Shock 70kDa protein 1B Human Recombinant ( HSPA1B Human )

Catalog # PT_73584

Print

$201.00
DescriptionThe HSPA1B Protein Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 650 amino acids having a molecular mass of 71.16kDa.HSPA1B is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary
Product Size: 2ug
Quantity

Data sheet

Formulation
The protein was filtered (0.4um) and lyophilized from a 0.5mg/ml solution containing 50mM phosphate buffer, 75mM NaCl, pH 7.4.
Solubility
It is recommended to add deionized water to prepare a working stock solution of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell cul
Inactivation
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program
Description
The HSPA1B Protein Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 650 amino acids having a molecular mass of 71.16kDa.HSPA1B is fused to a 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographi
Protein Background
The HSP70 family is found in many intracellular compartments such as chloroplasts, endoplasmic reticulum, mitochondria, and cytosol. These proteins are induced by a variation of biological stresses, including heat stress, in every organism. HSP70 has a ra
Expression host
Escherichia Coli.
Synonyms
Heat shock 70kDa protein 1B, HSP70-2, HSP70-1/HSP70-2, FLJ54328, HSPA1A, heat shock 70kDa protein 1A/1B, HSPA1.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized HSPA1B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSPA1B should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Amino acid sequence
MKHHHHHHAS AKAAAIGIDL GTTYSCVGVF QHGKVEIIAN DQGNRTTPSY VAFTDTERLI GDAAKNQVAL NPQNTVFDAK RLIGRKFGDP VVQSDMKHWP FQVINDGDKP KVQVSYKGET KAFYPEEISS MVLTKMKEIA EAYLGYPVTN AVITVPAYFN DSQRQATKDA GVIAGLNVLR IINEPTAAAI AYGLDRTGKG ERNVLIFDLG GGTFDVSILT IDDGIFEVKA TA
New