Intrinsic Factor Human Recombinant ( IF Human )

Data sheet

Formulation

The protein solution (0.7mg/ml) contains 20mM HEPES pH-8, 100mM NaCl, 20% Glycerol.

Purity

Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Intrinsic Factor Human Recombinant produced in baculovirus is a glycosylated, polypeptide chain containing having a molecular mass of 55,000 Dalton. The Intrinsic Factor is fused to a hexa-histidine at the C-terminus and purified by prop

Protein Background

Intrinsic Factor is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12 in the terminal ileum. Vitamin B12 is essential for e

Expression host

Sf9 Insect Cells.

Synonyms

Gastric intrinsic factor, Intrinsic factor, INF, IF, GIF, IFMH, TCN3, Cobalamin/Vitamin B-12 binding transport protein.

Reagent Appearance

Sterile Filtered pink solution. (The reactivity of the antigen is about 10 times higher when adding vitamin B12).

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.