Cytokeratin 8 Human Recombinant ( KRT8 Human )

Data sheet

Formulation

The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.

Solubility

It is recommended to reconstitute the lyophilized KRT8 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Cytokeratin 8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 53,532 Dalton. The KRT8 is purified by proprietary chromatographic techniques.

Protein Background

Keratin 8 and 18 (K8/18) are the major components of intermediate filament (IF) proteins of simple or single-layered epithelia.

Expression host

Escherichia Coli.

Synonyms

Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized KRT8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KRT8 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ca

References

1.Title:Role for the PP2A/B56 Phosphatase in Regulating 14-3-3 Release from Cdc25 to Control Mitosis.Publication:Copyright 2006 Elsevier Inc. All rights reserved.Cell, Volume 127, Issue 4, 759-773, 17 November 2006 doi:10.1016/j.cell.2006.10.035Link:htt