Lipopolysaccaride Human Recombinant ( LBP Human )

Data sheet

Formulation

Recombinant Human LBP was lyophilized from a protein solution (0.25 mg/ml) containing phosphate-buffered saline, pH 7.2.

Solubility

Every 10ug of recombinant human LBP should be reconstituted using 40ul of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.

Description

The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.The recombinant Human LBP was p

Protein Background

Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acu

Expression host

Chinese Hamster Ovarian Cells (CHO).

Synonyms

Lipopolysaccharide-binding protein, LBP, MGC22233.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Activity

Up to 0.2 ug/ ml LBP mediates binding of FITC-LPS (0.5ug/ml) to CD14+CHO transfectants (FACS).

Characterization

On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa.