Lipopolysaccaride Mouse Recombinant ( LBP Mouse )

Data sheet

Formulation

Recombinant Mouse LBP was lyophilized from a protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.

Solubility

It is recommended to reconstitute the lyophilized LBP in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Applications

ELISA Western Blotting.

Description

LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection the complete mouse LBP cDNA was amplified by PCR and cloned into expre

Protein Background

Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acu

Expression host

Chinese Hamster Ovarian Cells (CHO).

Synonyms

Lipopolysaccharide-binding protein, LBP, Ly88.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Characterization

On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa.