NANOG Human Recombinant ( NANOG Human )

Data sheet

Formulation

The NANOG solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 5% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

NANOG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 305 amino acids (1-305) and having a molecular mass of 34.6 kDa.The NANOG is purified by proprietary chromatographic techniques.

Protein Background

NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP.

Expression host

E.coli.

Synonyms

NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WN