NANOG-TAT Human Recombinant ( NANOG-TAT Human )

Data sheet

Formulation

NANOG was lyophilized after extensive dialysis against PBS.

Solubility

It is recommended to reconstitute the lyophilized NANOG in sterile PBS not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.

Description

NANOG Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 305 amino acids and having a molecular mass of 34.6kDa.The NANOG is fused to an N-terminal TAT (transcriptional activator protein) tag and purified by p

Protein Background

NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP.

Expression host

Escherichia Coli.

Synonyms

NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized NANOG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NANOG should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gly-Arg-Lys-Lys.