nudE Nuclear Distribution Gene E Homolog 1 Human Recombinant ( NDE1 Human )

Data sheet

Formulation

The NDE1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT and 0.2M NaCl.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

NDE1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 355 amino acids (1-335 a.a.) and having a molecular mass of 39.9kDa (Molecular weight on SDS-PAGE will appear higher).NDE1 is fused to a 20 amino acid His-tag at N-terminus

Protein Background

NDE1 is a cytoplasmic protein which is a member of the nudE protein family. Phosphorylated during mitosis, NDE1 is critical for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetoc

Expression host

Escherichia Coli.

Synonyms

Nuclear distribution protein nudE homolog 1, NudE, NDE1, NUDE, LIS4, NUDE1, HOM-TES-87.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

NDE1 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SV