Nucleopurin 210kDa Recombinant Human ( NUP210 Human )

Data sheet

Formulation

NUP210 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.

Purity

Greater than 80% as determined by SDS-PAGE.

Applications

Western blot with PBC sera or monoclonal anti-hexa-His-tag antibody.

Description

Recombinant NUP210 protein is a construct carrying multiple concatenated copies of the short cytoplasmic gp210 C-terminus (which contains the autoreactive epitopes) and having a molecular mass of 25,344 Dalton (pH 9.8). NUP210 protein is fused to a hexa-h

Protein Background

The NUP210 complex is a immense structure which spreads through the nuclear envelope, forming a doorway which regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the core components of the nuclear pore complex in e

Expression host

Sf9 insect cells.

Synonyms

Nucleoporin 210kDa, KIAA0906, GP210, Nuclear envelope pore membrane protein POM 210, Nuclear pore protein gp210, nuclear pore membrane glycoprotein 210.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Coating concentration

0.4-1.0 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.