Ras-Related C3 Botulinum Toxin Substrate 3 Human Recombinant ( RAC3 Human )

Data sheet

Formulation

The RAC3 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT, 200mM NaCl, 0.1mM PMSF and 1mM EDTA.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

RAC3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 189 amino acids (1-189 a.a.) and having a total molecular mass of 21kDa.RAC3 is purified by proprietary chromatographic techniques.

Protein Background

RAC3 belongs to the Rac subfamily of the Rho small G proteins. RAC3 is a small (approximately 21kDa) monomeric GTP-binding protein, which is an important component of intracellular signaling pathway, including the control of cell growth, cytoskeletal reor

Expression host

Escherichia Coli.

Synonyms

Ras-related C3 botulinum toxin substrate 3, p21-Rac3, RAC3.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

RAC3 although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Amino acid sequence

MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPHTPI LLVGTKLDLR DDKDTIERLR DKKLAPITYP QGLAMAREIG SVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKPGKKC.

Reconstitution to filaments

EAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYHHHHHH.