Ribosomal Phosphoprotein P0 Human Recombinant ( RPLP0 Human )

Data sheet

Formulation

RPLP0 is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA & 0.02% SDS.

Purity

Greater than 95% as determined by SDS-PAGE.

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & SLE sera (Systemic Lupus Erythematodes Disease).

Description

Ribosomal Phosphoprotein P0 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35,096 Dalton. RPLP0 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Protein Background

The ribosomal phosphoproteins, also called P protein antigens, are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35 kDa,

Expression host

Sf9 insect cells.

Synonyms

60S acidic ribosomal protein P0, Ribosomal Phosphoprotein P0, L10E, RPLP0, Ribosomal Protein Large P0, RPP0, P0, PRLP0, MGC88175, MGC111226.

Reagent Appearance

Sterile Filtered clear solution.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels.

Coating concentration

0.3-0.7 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.