Syntaphilin Human Recombinant ( SNPH Human )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The Syntaphilin protein solution contains 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol.

Solubility

This antibody will bind very well to protein A in a buffer (PBS) containing high salt concentration (3M Nacl).

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

SNPH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 444 amino acids (1-424) and having a molecular mass of 48.2 kDa.The SNPH is fused to 20 amino acid His-Tag at N-terminus and purified by standard chromato

Protein Background

Syntaxin-1, synaptobrevin, and SNAP25 cooperate to form the SNARE complex, which is needed for synaptic vesicle docking and fusion SNPH is a neuron-specific protein originally characterized as a binding partner of syntaxin-1. SNPH participates with SNAP25

Expression host

Escherichia Coli.

Synonyms

KIAA0374, MGC46096, bA314N13.5, SNPH, Syntaphilin.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

SNPH although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAMSLPGSRR TSAGSRRRTS PPVSVRDAYG TSSLSSSSNS GSYKGSDSSP TPRRSMKYTL CSDNHGIKPPTPEQYLTPLQ QKEVCIRHLK ARLKDTQDRL QDRDTEIDDL KTQLSRMQED WIEEECHRVE AQLALKEARK EIKQLKQVID TVKNNLIDKDKGLQKYFVDI NIQNKKLETL LHSMEVAQNG MAKEDGTGES AGGSPARSLT RSST

Antibody Properties

Reconstitute with 0.5ml H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson ImmunoResearch Laboratories) 1:10,000 dilution will yield 0.7 O.D within 10 minutes.