Streptavidin Recombinant, His Tag ( Streptavidin, His )

Data sheet

Formulation

The Streptavidin protein solution (1mg/ml) contains 20mM Tris-HCl pH7.5.

Purity

Greater than 95.0% as determined by analysis by SDS-PAGE.

Description

Recombinant Streptomyces Avidinii Streptavidin produced in E.Coli is a single, non-glycosylated polypeptide chain (25-183) containing a total of 167 amino acids and having a molecular mass of 17kDa. The Streptavidin protein is fused to an 8 aa N-terminal

Protein Background

Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-str

Expression host

Escherichia Coli.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ.

References

Title:Laterally mobile, functionalized self-assembled monolayers at the fluorous-aqueousinterface in a plug based microfluidic system: characterization and testing withmembrane protein crystallization.Publication:Department of Chemistry, The University of