Tropomyosin-4 Human Recombinant ( TPM4 Human )

Data sheet

Formulation

The TPM4 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

TPM4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 268 amino acids (1-248 a.a.) and having a molecular mass of 30.7kDa.TPM4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniq

Protein Background

TPM4 is a member of the tropomyosin family. Tropomyosins exist in practically all eukaryotic cells (both muscle and nonmuscle), where they bind actin filaments and function to modulate actin-myosin interaction and stabilize actin filament structure. TPM4

Expression host

Escherichia Coli.

Synonyms

Tropomyosin alpha-4 chain, TM30p1, Tropomyosin-4, TPM4.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAGLNSLEAV KRKIQALQQQ ADEAEDRAQG LQRELDGERE RREKAEGDVA ALNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRAMK DEEKMEIQEM QLKEAKHIAE EADRKYEEVA RKLVILEGEL ERAEERAEVS ELKCGDLEEE LKNVTNNLKS LEAASEKYSE KEDKYEEEIK LLSDKLKEAE TRAEFAERTV AK